Metabolomics approach based on LC-HRMS for the fast screening of iron(II)-chelating peptides in protein hydrolysates

Production of iron-chelating peptides from protein hydrolysates requires robust and adequate screening methods to optimize their purification subsequently valorize potential antioxidant properties. An original methodology was developed for direct sensitive iron(II)-chelating based on ion-pair reverse phase liquid chromatography (IP-RPLC) coupled high-resolution mass spectrometry (HRMS). Peptide mixture first added iron(II) solution form iron(II)-peptide complexes. Then IP-RPLC-HRMS analysis conducted this iron-peptide the iron-free peptide comparative spectra analysis. This protocol, initially applied a range low molecular weight standard peptides, allowed detection [(Peptide-H)+56FeII]+ complex ion (GGH, EAH, DAH, ?AH, DMH, DTH, DSH). GGH in mixtures targeted [(GGH-H)+56FeII]+ showed limit (LOD) below 0.77 mg L?1 GGH. protocol finally tested combination with metabolomics software additional digital processing non-targeted search peptides. Applicability new has been validated by as when at casein hydrolysate.